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Representative two-dimensional gel electrophoresis of rainbow trout liver proteins . Total liver protein extract (150 μg) was separated first by IEF on <t>3–10</t> <t>non-linear</t> <t>IPG</t> drystrips, and then SDS-PAGE was performed on 12.5% polyacrylamide gel. The proteins were revealed by silver staining as described by Heukeshoiven and Dernick (1985). Differentially abundant proteins positively identified by trypsin digest fingerprinting are located by arrows, with their corresponding identity and spot ID.
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Representative two-dimensional gel electrophoresis of rainbow trout liver proteins . Total liver protein extract (150 μg) was separated first by IEF on 3–10 non-linear IPG drystrips, and then SDS-PAGE was performed on 12.5% polyacrylamide gel. The proteins were revealed by silver staining as described by Heukeshoiven and Dernick (1985). Differentially abundant proteins positively identified by trypsin digest fingerprinting are located by arrows, with their corresponding identity and spot ID.

Journal: BMC Genomics

Article Title: Changes induced by dietary energy intake and divergent selection for muscle fat content in rainbow trout ( Oncorhynchus mykiss ), assessed by transcriptome and proteome analysis of the liver

doi: 10.1186/1471-2164-9-506

Figure Lengend Snippet: Representative two-dimensional gel electrophoresis of rainbow trout liver proteins . Total liver protein extract (150 μg) was separated first by IEF on 3–10 non-linear IPG drystrips, and then SDS-PAGE was performed on 12.5% polyacrylamide gel. The proteins were revealed by silver staining as described by Heukeshoiven and Dernick (1985). Differentially abundant proteins positively identified by trypsin digest fingerprinting are located by arrows, with their corresponding identity and spot ID.

Article Snippet: Samples (150 μg total proteins) were diluted to 450 μL with DeStreak solution supplemented with 0.5% IPG buffer pH 3–10 NL (Amersham Biosciences).

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, SDS Page, Silver Staining